Faster enzymatic procedure for serum triglycerides.
نویسندگان
چکیده
We describe a single-reagent, enzymatic procedure for triglycerides in serum, which may be performed in 5 min per sample with use of an Abbott ABA-100 analyzer system. This procedure is compared to the semi-automated enzymatic procedure with the ABA-100, which requires 30 min per sample.
منابع مشابه
Evaluation of a new enzymatic kit for serum triglycerides.
We evaluated a new enzymatic kit for serum triglycerides (triacylglycerols) in two laboratories. The procedure involved both enzymatic hydrolysis of the triglycerides and conversion of the glycerol, which was linked to an indicator reaction. There was excellent agreement with the mechanized procedure of Kessler and Lederer and with a manual method. The test requires 50 mul of serum and can be c...
متن کاملQuantitative determination of serum triglycerides by the use of enzymes.
We describe a novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure. Under the conditions of the assay, the enzymatic hydrolysis can be completed in less than 10 mm by the combined action of a microbial lipase and a protease. We have been able to demonstrate complete hydrolysis of triglycerides by thin-layer c...
متن کاملDetermination of serum triglycerides by an accurate enzymatic method not affected by free glycerol.
In this automated single-run enzymatic procedure for specific determination of triglycerides in serum, free glycerol is removed from the reaction mixture by pre-incubation with glycerol phosphate oxidase and peroxidase. The subsequent addition of lipase and the chromogen, 4-aminoantipyrine, results in the formation of color proportional to the amount of triglycerides in serum. Standards contain...
متن کاملSimplified, totally enzymatic method for determination of serum triglycerides with a centrifugal analyzer.
We describe a totally enzymatic method for determination of serum triglycerides (triacylglycerols) specifically adaptable to the CentrifiChem system. The method involves lipolysis with lipase from Rhizopus arrhizus alone and quantitation of the resulting glycerol with glycerol dehydrogenase in a kinetic, fixed-time mode. Hydrolysis by the lipase is complete, for concentrations up to at least 5....
متن کاملAn AutoAnalyzer method for estimating serum glyceride glycerol using a glycerokinase procedure.
THE GLYCERIDE GLYCEROL ANALYSIS DEPENDS, AFTER SAPONIFICATION OF TRIGLYCERIDES, ON A LINKED ENZYMATIC PROCEDURE USING GLYCEROKINASE, PYRUVATE KINASE, AND LACTATE DEHYDROGENASE: the final conversion of NADH to NAD(+) is followed fluorimetrically. Twenty analyses can be performed per hour on the AutoAnalyzer; recoveries of added triglycerides ranged between 90 and 104%. In a mixed male and female...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 21 13 شماره
صفحات -
تاریخ انتشار 1975